Inducible and constitutive nucleoside-binding sites in Escherichia coli: differential inhibition by nucleoside analogues

Inducible and constitutive nucleoside-binding sites in Escherichia coli: differential inhibition by nucleoside analogues.

Constitutive and inducible nucleoside-binding sites on the surface of the cells of E. coli B have different patterns of inhibition by nucleoside analogues. 1(beta-D-Ribofuranosyl)-4-aminopyrimidine-6-one and isoguanosine preferentially inhibit the inducible binding sites while showdomycin and N(4)-dimethylcytidine interfere more strongly with the constitutive function.

Inhibition of nucleoside-binding sites by nucleoside analogues in Escherichia coli.

SUMMARY Nucleoside analogues modified in heteroeyclic bases or sugar moieties were screened for their ability of counteracting the bacteriostatic effect of showdomycin in E. colio Pyrimidine ribonucleosides or 2-deoxyribonucleosides substituted at the position 5 wijh halogen^atom, methyl or hydroxyl group, 2-fluoro-, 2-chloro-2-deoxyuridme and its 5-methyl homologue, N^-dimethylcytidine and N*-...

Hydroxyurea and Escherichia coli nucleoside diphosphate reductase.

M). The cultures were incubated at 37° for 40 mm (“Pretreatment―), whereupon the bacteria were washed and resuspended in fresh Medium HA. Portions from each culture were distributed into flasks containing premeasured amounts of the various drugs (“Additions―). After receiving cytidine-3H (2 @ig/ml; 0.13 i@Ci/ml),the cultures were incubated with aeration at 37°for 20 mm and quadr.uplic...

Coupled nucleoside phosphorylase reactions in Escherichia coli.

Chemical Synthesis of Nucleoside Analogues Chemical Synthesis of Nucleoside Analogues